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MMP19 - catalytic domain
貨號：G04MP19C
品牌：Giotto Biotech
規(guī)格：10 μg
目錄價(jià)：詢價(jià)
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Description

Description
MW = 17.6 kDa calculated. Matrix Metalloproteinase-19 isoform 1 RASI-1 (MMP-19, Matrix Metalloproteinase RASI, MMP-18) catalytic domain cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-19, (residues 101-256, UniProtKB accession Q99542).
 
Sequence 
                  110        120        130        140
        M- LGRWRKKHLT FRILNLPSTL PPHTARAALR QAFQDWSNVA
       150        160        170        180        190 
PLTFQEVQAG AADIRLSFHG RQSSYCSNTF DGPGRVLAHA DIPELGSVHF 
       200        210        220        230        240 
DEDEFWTEGT YRGVNLRIIA AHEVGHALGL GHSRYSQALM APVYEGYRPH 
       250
FKLHPDDVAG IQALYG
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
 
Supplied as
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.2 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 25440 M-1cm-1 calculated).

Specific activity
> 1.5 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric ***** with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate. 

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.